Solvent production from rice straw by a co-culture of Clostridium acetobutylicum and Saccharomyces cerevisiae: effect of pH control

Abstract One of the challenges in biofuel production from lignocellulosic wastes is to improve its conversion solvents; therefore, new strategies enhance xylose uptake are required due be secondary abundant sugar. In this context, a novel fermentation strategy integrating co-culture Clostridium acetobutylicum and Saccharomyces cerevisiae with pH control was developed. Initially, two different buffers, ammonium acetate calcium carbonate, were tested under min > 4.8 by fermenting 60 g L −1 glucose C. monoculture. Ammonium selected for media as butanol increased 9.8 10.9 over carbonate test. Comparing spontaneous acetone-butanol-ethanol (ABE) when no consumption observed, efficiently controlling 4.8. The 47% either using 45:15 glucose:xylose mixture or rice straw (RS) hydrolysate. monoculture RS hydrolysate produced concentration 6.5 (9.5) . While it 7.0 (13.1) S. used same regulation mainly ethanol increase up 2.7 Moreover, doubled 94% amino-acid secretion yeast. Overall, combined very effective method promoting sugar ABE solvent waste.